Ah, PCR has three main steps, na? Denaturation at 94-96°C to split the DNA, annealing around 50-65°C for primers to bind, and extension at 72°C for the Taq polymerase to work. But in our lab, we are always tweaking the annealing temperature—too precise and the primer won’t catch, too loose and you get nonsense bands. I know this well, because learning these temperature curves on the new automated cyclers felt like teaching an old dog new tricks, and I saw the junior staff smirking when I took notes.
#javelin#throws
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