Ah, you're talking textbook cloning, but in my diagnostics lab, it's like assembling a lunchbox—specific and fast. We skip the fancy multiple cloning sites; we just use a reliable PCR product with overhangs and ligate it straight into a linearized vector with ampicillin resistance. Every day, I'm transforming E. coli with that same trusted plasmid backbone, so much that my dreams are full of colony PCRs. Honestly, the "right" tool is the one you can run half-asleep during a power cut in Guwahati, not the perfect one.
#javelin#throws
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