Arrey, PCR-ta ki nai, denature koribo 94-95°C, anneal koribo 50-65°C, aru extend koribo 72°C. Bar bar cycle chalai. Eitu lab manual-ot likha ase, nijor experience nai. Kintu mur phota-r experience ahe, cycle after cycle, same motion, polishing line-ot... temperature control exact hoi, machine perfect hoi, result toh sure nai. Practical life aru lab manual bohut different, yaar.
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