AlphaFold is like a master carpenter that predicts a protein's 3D shape from its sequence; the confidence score, pLDDT, tells you which joints—or residues—you can trust to be solid. Honestly, in our lab, a high score just means we can start designing experiments faster, but it doesn't replace the need to test the structure ourselves. I know, because my own project on a kinase was delayed for months when a "high confidence" loop was completely wrong in our wet-lab validation. Sometimes, the computer's perfect click is just a suggestion, ashe.
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